As our heroes stare in amazement at the Violet City gym, Jessie and James, in a rather pathetic disguise, make their appearance pretending to be conducting a survey on behalf of the Pokémon Bureau. Although Ash, Misty and Brock have never heard of it, they let Jessie examine Pikachu to see if he meets the official Violet City gym standards. Jessie and James confiscate Pikachu and run off.
A young boy named Ash Ketchum embarks on a journey to become a "Pokemon Master" with his first Pokemon, Pikachu. Joining him on his travels are Brock, a girl-obsessed Rock Pokemon Trainer, and Misty, a tomboyish Water Pokemon Trainer who may have a crush on him. Ash and Co. end up traveling through various regions, including Kanto, the Orange Islands, and Johto, and then enter the Pokemon League competitions there.
Along the way, they run into many confrontations with Jessie, James, and Meowth, a trio of Pokemon thieves who are apart of an evil organization called "Team Rocket". But every time Team Rocket try to do their evil deeds, they fail thanks to Ash and his Pokemon.